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[email protected]We examined potassium channel gene expression of two members of the Shaker subfamily, MK1 and MK2, in sciatic nerves from rats and mice. In Northern blot analysis, MK1 and MK2 probes detected single transcripts of approximately 8 kb and approximately 9.5 kb, respectively, in sciatic nerve and brain from both species.
Get PriceWe show that mceph/mceph mice carry an 11-base-pair deletion in the gene encoding the Shaker-like voltage-gated potassium channel subtype 1, Kcna1. The mutation leads to a frame shift and the predicted MCEPH protein is truncated at amino acid 230 (out of 495), terminating with six aberrant amino acids. The expression of Kcna1 mRNA is increased ...
Get PriceThe KCNA1 gene belongs to a large family of genes that provide instructions for making potassium channels. These channels, which transport positively charged atoms (ions) of potassium into and out of cells, play a key role in a cell's ability to generate and transmit electrical signals. The KCNA1 gene provides instructions for making one part ...
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Get PriceMar 24, 2004 Shaker potassium channel complexes are thought to be composed of 4 alpha and 4 beta subunits. Cloning and Expression By PCR of a human hippocampal library with degenerate primers based on conserved regions of rat beta-1 (KCNA1B; 601141) and beta-2 subunits, McCormack et al. (1995) isolated cDNAs encoding human beta-1 and beta-2.
Get PriceApr 04, 2006 Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Forms rapidly inactivating tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient and …
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Get PriceProperties of shaker-type potassium channels in higher plants J Membr Biol. 2006 Mar;210(1):1-19. doi: 10.1007/s00232-006-0856-x. Epub 2006 Jun 22. Authors F Gambale 1 , N Uozumi. Affiliation 1 Istituto di Biofisica, Consiglio ...
Get PriceThe conformational changes associated with activation gating in Shaker potassium channels are functionally characterized in patch-clamp recordings made from Xenopus laevis oocytes expressing Shaker channels with fast inactivation removed. Estimates of the forward and backward rates for transitions are obtained by fitting exponentials to macroscopic ionic and gating current relaxations at ...
Get PriceJan 24, 1991 Cite this article. Papazian, D., Timpe, L., Jan, Y. et al. Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence.Nature 349 ...
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